hap1 cells (OriGene)
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Hap1 Cells, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hap1+cells/Apex1+(BC052401)+Mouse+Untagged+Clone/bio_rxiv__2025__01__09__632087-79-0-19
Average 93 stars, based on 54 article reviews
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Plasmid Preparation:Article Title: Ibetazol, a novel inhibitor of importin β1-mediated nuclear import Article Snippet: Phosphorotioate bonds were incorporated into the primers during synthesis by the manufacturer (IDT). .. Article Title: Ibetazol, a novel inhibitor of importin β1-mediated nuclear import Article Snippet: pCMV_AncBE4max_P2A_GFP and pCMV_ABEmax_P2A_GFP were a gift from David Liu (Addgene plasmid #112100 and #112101 ). .. Knock-In:Article Title: Ibetazol, a novel inhibitor of importin β1-mediated nuclear import Article Snippet: Phosphorotioate bonds were incorporated into the primers during synthesis by the manufacturer (IDT). .. Transfection:Article Title: Ibetazol, a novel inhibitor of importin β1-mediated nuclear import Article Snippet: pCMV_AncBE4max_P2A_GFP and pCMV_ABEmax_P2A_GFP were a gift from David Liu (Addgene plasmid #112100 and #112101 ). .. Article Title: Ibetazol, a novel inhibitor of importin β1-mediated nuclear import Article Snippet: .. Briefly, Hela Kyoto cells were transfected using Lipofectamine 3000 Transfection Reagent (ThermoFisher scientific L3000001) and Article Title: AMPylation regulates PLD3 processing Article Snippet: .. Article Title: Saturation genome editing-based functional evaluation and clinical classification of BRCA2 single nucleotide variants Article Snippet: For haploidy sorting, 1X10-7 HAP1 cells were resuspend in 5ug/ml Hoechst 34580 (BD, 565877) and sorted at 4°C. .. Article Title: Ibetazol, a novel inhibitor of importin β1-mediated nuclear import. Article Snippet: Nucleocytoplasmatic transport plays an essential role in eukaryotic cell homeostasis and is mediated by karyopherins.. Importin β1 (KPNB1) and its adaptor protein importin α1 (KPNA2) are the bestcharacterized karyopherins that effect nuclear import.. Here, we identify a novel small-molecule inhibitor of the importin β1-mediated nuclear import. Article Title: Functional evaluation and clinical classification of BRCA2 variants. Article Snippet: For haploidy sorting, 1 × 10−7 HAP1 cells were resuspended in 5 mg ml–1 Hoechst 34580 (BD, 565877) and sorted at 4 °C. .. |

